Human Growth Hormone Inducible Transmembrane Protein (GHITM) ELISA Kit
The Human Growth Hormone-Inducible Transmembrane Protein (Ghitm) ELISA Kit is a cutting-edge tool for the precise measurement of Ghitm levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of experimental settings.Ghitm is a pivotal protein that is implicated in cell growth and differentiation processes, making it a key player in various physiological and pathological conditions. By measuring Ghitm levels, researchers can gain valuable insights into the mechanisms underlying growth regulation and potentially identify novel therapeutic targets for diseases related to abnormal growth signaling pathways.
Whether investigating mechanisms of growth regulation, exploring potential therapeutic interventions, or monitoring disease progression, the Human Ghitm ELISA Kit provides researchers with a reliable and efficient solution for studying the role of Ghitm in human health and disease. Order yours today and unlock the potential of Ghitm research in your lab.
Product Name:
Human Growth Hormone Inducible Transmembrane Protein (GHITM) ELISA Kit
Product Code:
HUDL01183
Size:
96 Assays
Target:
GHITM
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
