The Human GZMB (Granzyme B) ELISA Kit is specifically designed for the precise measurement of granzyme B levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable tool for various research purposes.Granzyme B is a vital enzyme involved in immune responses, particularly cytotoxic T cell-mediated apoptosis. It plays a crucial role in immune surveillance, defense against infections, and anti-tumor immunity. Monitoring granzyme B levels can provide valuable insights into the immune system function and aid in the development of immunotherapies for cancer and other diseases.Get your Human GZMB ELISA Kit today and unlock the potential for groundbreaking discoveries in immunology and cancer research.
Product Name:
Human GzmB (Granzyme B) ELISA Kit
SKU:
HUES02621
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
