Description
Human H2AX Recombinant Protein (N-His)
Human H2AX Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Tyr143, with an N-terminal His tag. Predicted molecular weight: 17.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Histone H2AX is a ~15 kDa protein. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. For research use only.
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
| Product Name: | Human H2AX Recombinant Protein (N-His) |
| SKU: | GXRP01029 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | H2a/x, Histone H2A.X, H2AFX, Histone H2AX, H2AX |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Tyr143 |
| Calculated MW: | 17.31 kDa |
| UniProt: | P16104 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
