The Human HB (Hemoglobin) ELISA Kit is specially designed to accurately measure levels of hemoglobin in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable tool for various research purposes.Hemoglobin is a vital protein responsible for carrying oxygen in the blood, and abnormalities in its levels can indicate various health conditions such as anemia, sickle cell disease, and other blood disorders. By using this ELISA kit, researchers can gain valuable insights into these conditions and potentially develop new treatment strategies.Overall, the Human HB (Hemoglobin) ELISA Kit is a reliable and efficient tool for studying hemoglobin levels in human samples, offering researchers a comprehensive solution for their investigative needs.
Product Name:
Human HB (Hemoglobin) ELISA Kit
SKU:
HUES01628
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
