Description
Human HDAC3 Recombinant Protein (N-His)
Human HDAC3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Gln376, with an N-terminal His tag. Predicted molecular weight: 45.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Histone deacetylase 3 (HDAC3) is a ~48 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. For research use only.
Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
| Product Name: | Human HDAC3 Recombinant Protein (N-His) |
| SKU: | GXRP00120 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | HD3, SMAP45, RPD3-2, Histone deacetylase 3, HDAC3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Gln376 |
| Calculated MW: | 45.03 kDa |
| UniProt: | O15379 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
