Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit
The Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit is a cutting-edge tool designed for the precise measurement of HDGF levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research purposes. HDGF is a vital protein known for its role in cellular growth and proliferation, making it a key player in processes like tissue regeneration and development. Its implications in diseases such as hepatocellular carcinoma and other malignancies highlight the significance of HDGF as a valuable biomarker for disease research and potential therapeutic interventions.
With the Human HDGF ELISA Kit, researchers can delve deeper into the intricate mechanisms involving HDGF, paving the way for new insights and advancements in biomedical research. Trust in the reliability and effectiveness of this kit to drive your investigations forward and uncover new discoveries in the field of molecular biology.
Product Name:
Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit
SKU:
HUFI07820
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
99-104%
87-98%
82-96%
EDTA Plasma (n = 5)
83-101%
84-97%
87-97%
Heparin Plasma (n = 5)
84-100%
81-97%
81-99%
Recovery:
Sample
1
2
Serum (n = 5)
85-100
92
EDTA Plasma (n = 5)
88-103
95
Heparin Plasma (n = 5)
89-105
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
