The Human HDLBP/Vigilin ELISA Kit is a powerful tool for the precise measurement of Human HDLBP/Vigilin levels in various biological samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it an invaluable asset for a diverse array of research applications. HDLBP/Vigilin is a critical protein that plays a key role in RNA metabolism and transport, as well as in the regulation of gene expression. Dysregulation of HDLBP/Vigilin has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
As a result, HDLBP/Vigilin serves as a vital biomarker for studying these conditions and exploring potential therapeutic interventions. Incorporating the Human HDLBP/Vigilin ELISA Kit into your research workflow will enable you to accurately quantify HDLBP/Vigilin levels, providing valuable insights into the mechanisms underlying disease pathology and potential treatment strategies. Trust in the reliability and precision of this ELISA kit to accelerate your research efforts and drive meaningful discoveries in the realm of human biology.
Product Name:
Human HDLBP/Vigilin ELISA Kit
SKU:
HUFI02148
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-101%
88-101%
82-96%
EDTA Plasma (n = 5)
87-105%
85-101%
88-97%
Heparin Plasma (n = 5)
86-105%
82-100%
80-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-102
91
EDTA Plasma (n = 5)
88-101
96
Heparin Plasma (n = 5)
86-97
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
