Human Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
The Human Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit is a cutting-edge tool designed for the accurate quantification of HSPB1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.HSPB1, also known as heat shock 27kDa protein 1, is a key player in cellular stress response and is involved in protecting cells from damage and promoting cell survival. Dysregulation of HSPB1 has been linked to various diseases, including neurodegenerative disorders, muscle diseases, and cardiovascular conditions.
Thus, the measurement of HSPB1 levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Human HSPB1 ELISA Kit offers researchers a reliable and efficient tool for studying the role of HSPB1 in health and disease, making it a valuable addition to any laboratory focused on cellular stress response and protein biomarker research.
Product Name:
Human Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
Product Code:
HUDL01390
Size:
96 Assays
Target:
HSPB1
Synonyms:
HSP27, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock Protein 27, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
