SynonymsMIF2,BIP,GRP78,Immunoglobulin heavy chain-binding protein,Glucose Regulated Protein 78,Binding Immunoglobulin Protein,Endoplasmic reticulum lumenal Ca binding grp78
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL01388
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11021
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MIF2,BIP,GRP78,Immunoglobulin heavy chain-binding protein,Glucose Regulated Protein 78,Binding Immunoglobulin Protein,Endoplasmic reticulum lumenal Ca binding grp78
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit
The Human Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit is a powerful tool for the accurate measurement of HSPA5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and consistent results for a variety of research applications.HSPA5, also known as BiP or GRP78, is a key molecular chaperone involved in protein folding and quality control in the endoplasmic reticulum. It plays a crucial role in cellular stress responses, as well as in regulating cell survival and apoptosis.
Dysregulation of HSPA5 has been implicated in various diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human HSPA5 ELISA Kit offers researchers a precise and efficient way to measure HSPA5 levels in human samples, allowing for deeper insights into the role of this important protein in health and disease.
Product Name:
Human Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit
Product Code:
HUDL01388
Size:
96 Assays
Target:
HSPA5
Synonyms:
MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
