SynonymsKIM-3,TIM3,TIMD3,Tim-3,T-cell membrane protein 3,T-cell immunoglobulin mucin receptor 3,T-cell immunoglobulin and mucin domain-containing protein 3
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL01304
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8TDQ0
Sensitivity:
36pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
KIM-3,TIM3,TIMD3,Tim-3,T-cell membrane protein 3,T-cell immunoglobulin mucin receptor 3,T-cell immunoglobulin and mucin domain-containing protein 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Hepatitis A Virus Cellular Receptor 2 (HAVCR2) ELISA Kit
The Human Hepatitis A Virus Cellular Receptor 2 (HAVCR2) ELISA Kit is a state-of-the-art tool for precise measurement of HAVCR2 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable asset for various research purposes.HAVCR2, also known as TIM-3, is a key receptor involved in immune responses and regulation of T cell activation.
Its dysregulation has been linked to various diseases, including viral infections and autoimmune disorders, making it a valuable target for therapeutic interventions. The HAVCR2 ELISA Kit provides researchers with a reliable means of quantifying HAVCR2 levels, enabling a deeper understanding of its role in disease pathogenesis and potential therapeutic strategies.
Product Name:
Human Hepatitis A Virus Cellular Receptor 2 (HAVCR2) ELISA Kit
Product Code:
HUDL01304
Size:
96 Assays
Target:
HAVCR2
Synonyms:
KIM-3, TIM3, TIMD3, Tim-3, T-cell membrane protein 3, T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
