Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit
The Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit is a reliable and accurate tool for measuring HGFR levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity to ensure precise and reproducible results for various research applications.HGFR, also known as c-Met, is a receptor essential for cell growth, survival, and migration. It plays a key role in various cellular processes, including tissue regeneration, wound healing, and tumorigenesis.
Dysregulation of HGFR signaling has been implicated in cancer progression, making it a valuable biomarker for cancer research and therapy development.With the Human HGFR ELISA Kit, researchers can confidently study the role of HGFR in different physiological and pathological conditions, contributing to a better understanding of its function and potential therapeutic implications. Trust in the accuracy and reliability of this ELISA kit for your research needs.
Product Name:
Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit
Product Code:
HUDL01332
Size:
96 Assays
Target:
HGFR
Synonyms:
CMET, RCCP2, C-Met, Met Proto-Oncogene, Mesenchymal-Epithelial Transition Factor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
