Description
Human HEXB Recombinant Protein (N-His)
Human HEXB Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys315-Tyr456, with an N-terminal His tag. Predicted molecular weight: 18.57 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Beta-hexosaminidase subunit beta (HEXB/HCC7) is a ~63 kDa protein. Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. For research use only.
The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
| Product Name: | Human HEXB Recombinant Protein (N-His) |
| SKU: | GXRP00679 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | N-Acetyl-β-glucosaminidase (NAG), chitobiase, NAG, Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, N-acetyl-beta-glucosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, Hexosaminidase subunit B, HCC-7, HEXB |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys315-Tyr456 |
| Calculated MW: | 18.57 kDa |
| UniProt: | P07686 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
