Human HIF-1AN / HIF-1 alpha inhibitor ELISA Kit
- SKU:
- HUFI00694
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q9NWT6
- Sensitivity:
- 0.188ng/ml
- Range:
- 0.313-20ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- HIF1AN, FIH-1, Hypoxia-inducible factor 1-alpha inhibitor, HIF-1AN, Peptide-aspartate beta-dioxygenase, Factor inhibiting HIF-1, FIH1, hypoxia inducible factor 1, alpha subunit inhibitor, Hypoxia-inducible factor asparagine hydroxylase
- Reactivity:
- Human
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
Human HIF-1AN / HIF-1 alpha inhibitor ELISA
HIF-1AN / HIF-1 alpha inhibitor is an orally bioavailable small molecule that exhibits specific inhibition of the transcriptional activity of HIF-1. HIF-1AN / HIF-1 alpha inhibitor has been shown to inhibit hypoxia induced increase in VEGF secretion, angiogenesis and cell proliferation by interfering with the function of HIF-1alpha. HIF-1AN / HIF-1 alpha inhibitor is ideal for targeting angiogenesis associated with diseases such as cancer or macular degeneration. Inhibition of HIF-1 for therapeutic purposes has also been tested in models of ischemic diseases and Alzheimer's disease.
| Product Name: | Human HIF-1AN / HIF-1 alpha inhibitor ELISA Kit |
| Product Code: | HUFI00694 |
| Size: | 96 Assays |
| Alias: | HIF1AN, FIH-1, Hypoxia-inducible factor 1-alpha inhibitor, HIF-1AN, Peptide-aspartate beta-dioxygenase, Factor inhibiting HIF-1, FIH1, hypoxia inducible factor 1, alpha subunit inhibitor, Hypoxia-inducible factor asparagine hydroxylase |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human HIF1AN concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human HIF1AN and the recovery rates were calculated by comparing the measured value to the expected amount of Human HIF1AN in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human HIF1AN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | Q9NWT6 |
| UniProt Protein Function: | HIF1AN: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4. |
| UniProt Protein Details: | Protein type:EC 1.14.11.30; EC 1.14.11.n4; Oxidoreductase Chromosomal Location of Human Ortholog: 10q24 Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus Molecular Function:NF-kappaB binding; protein binding; protein homodimerization activity; oxygen sensor activity; zinc ion binding; carboxylic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; Notch binding; cofactor binding Biological Process: peptidyl-aspartic acid hydroxylation; transcription, DNA-dependent; peptidyl-asparagine hydroxylation; negative regulation of Notch signaling pathway; positive regulation of myoblast differentiation |
| UniProt Code: | Q9NWT6 |
| NCBI GenInfo Identifier: | 32129605 |
| NCBI Gene ID: | 55662 |
| NCBI Accession: | Q9NWT6.2 |
| UniProt Secondary Accession: | Q9NWT6,Q5W147, Q969Q7, Q9NPV5, D3DR69, |
| UniProt Related Accession: | Q9NWT6 |
| Molecular Weight: | 40,285 Da |
| NCBI Full Name: | Hypoxia-inducible factor 1-alpha inhibitor |
| NCBI Synonym Full Names: | hypoxia inducible factor 1, alpha subunit inhibitor |
| NCBI Official Symbol: | HIF1AN |
| NCBI Official Synonym Symbols: | FIH1 |
| NCBI Protein Information: | hypoxia-inducible factor 1-alpha inhibitor; FIH-1; factor inhibiting HIF-1; factor inhibiting HIF1; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase |
| UniProt Protein Name: | Hypoxia-inducible factor 1-alpha inhibitor |
| UniProt Synonym Protein Names: | Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase |
| Protein Family: | Hypoxia-inducible factor 1-alpha inhibitor |
| UniProt Gene Name: | HIF1AN |
| UniProt Entry Name: | HIF1N_HUMAN |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Protocol |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
| 6. | Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2. |
| 7. | Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall. |
| 8. | Seal the plate with a cover and incubate at 37°C for 60 min. |
| 9. | Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash. |
| 10. | Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min. |
| 11. | Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min. |
| 12. | Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color. |
| 13. | Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately. |
| 14. | Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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