Human High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit
The Human High Mobility Group (AT-Hook) Protein 1 (HMGA1) ELISA Kit is specifically designed for the precise detection of HMGA1 levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research applications.HMGA1 is a vital protein that plays a key role in regulating gene expression and influencing cell growth and differentiation. Dysregulation of HMGA1 has been linked to various diseases, including cancer, diabetes, and autoimmune disorders, making it a valuable biomarker for studying disease mechanisms and developing potential therapeutic interventions.
The HMGA1 ELISA Kit from Assay Genie provides researchers with a reliable tool for quantifying HMGA1 levels in biological samples, allowing for in-depth analysis of HMGA1 function and its implications in disease pathology. Trust in Assay Genie's expertise and high-quality products to advance your research in the field of molecular biology and beyond. [Note: This is a fictional product description created by the AI based on the provided URL and template. It may not accurately represent the actual product on the website.]
Product Name:
Human High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit
Product Code:
HUDL01341
Size:
96 Assays
Target:
HMGA1
Synonyms:
HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
