Human Histone Acetyltransferase 1 (HAT1) ELISA Kit
The Human Histone Acetyltransferase 1 (HAT1) ELISA Kit is a powerful tool for accurately measuring levels of HAT1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and consistent results, making it perfect for a variety of research applications.HAT1 is an important enzyme involved in the process of histone acetylation, which plays a crucial role in regulating gene expression and chromatin structure. Dysregulation of HAT1 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its significance as a potential therapeutic target and biomarker for these conditions.
With the Human HAT1 ELISA Kit, researchers can accurately quantify HAT1 levels in human samples, providing valuable insights into its role in various biological processes and diseases. This kit is a valuable resource for studying epigenetic mechanisms and developing novel therapeutic strategies.
Product Name:
Human Histone Acetyltransferase 1 (HAT1) ELISA Kit
Product Code:
HUDL01303
Size:
96 Assays
Target:
HAT1
Synonyms:
KAT1, Histone acetyltransferase type B catalytic subunit
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
