Human HLA-DPB1 Recombinant Protein (N-His) (GXRP07641)

Human HLA-DPB1 Recombinant Protein (N-His)

Human HLA-DPB1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn35-Gln218, with an N-terminal His tag. Predicted molecular weight: 23.76 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. HLA class II histocompatibility antigen, DP beta 1 chain (HLA-DPB1) is a ~29 kDa protein. Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. For research use only.

The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components.