Description
Human HLTF Recombinant Protein (N-His)
Human HLTF Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala837-Lys996, with an N-terminal His tag. Predicted molecular weight: 20.01 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF is a ~113 kDa protein. Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT). For research use only.
Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching. Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance: specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Catalyzes formation of H3K23ub in two steps: first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2. Also acts as a chromatin redodeling factor, thereby regulating transcription.
| Product Name: | Human HLTF Recombinant Protein (N-His) |
| SKU: | GXRP03472 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | HIP116, RING-type E3 ubiquitin transferase HLTF, SNF2L3, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HLTF, Sucrose nonfermenting protein 2-like 3, ZBU1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Helicase-like transcription factor, RING finger protein 80, SMARCA3, HIP116A, RNF80 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala837-Lys996 |
| Calculated MW: | 20.01 kDa |
| UniProt: | Q14527 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
