Description
Human HMBS Recombinant Protein (N-His)
Human HMBS Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu85-Ser337, with an N-terminal His tag. Predicted molecular weight: 30.08 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Porphobilinogen deaminase (HMBS) is a ~39 kDa protein. As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. For research use only.
Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact.
| Product Name: | Human HMBS Recombinant Protein (N-His) |
| SKU: | GXRP00717 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Hydroxymethylbilane synthase, PBG-D, Pre-uroporphyrinogen synthase, Porphobilinogen deaminase, PBGD, UPS, HMBS |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu85-Ser337 |
| Calculated MW: | 30.08 kDa |
| UniProt: | P08397 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
