Description
Human HSD11B2 Recombinant Protein (N-His)
Human HSD11B2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly106-Arg405, with an N-terminal His tag. Predicted molecular weight: 35.15 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 11-beta-hydroxysteroid dehydrogenase type 2 (HSD11B2) is a ~44 kDa protein. Catalyzes the conversion of biologically active 11beta-hydroxyglucocorticoids (11beta-hydroxysteroid) such as cortisol, to inactive 11-ketoglucocorticoids (11-oxosteroid) such as cortisone, in the presence of NAD(+). For research use only.
Functions as a dehydrogenase (oxidase), thereby decreasing the concentration of active glucocorticoids, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Plays an important role in maintaining glucocorticoids balance during preimplantation and protects the fetus from excessive maternal corticosterone exposure. Catalyzes the oxidation of 11beta-hydroxytestosterone (11beta,17beta-dihydroxyandrost-4-ene-3-one) to 11-ketotestosterone (17beta-hydroxyandrost-4-ene-3,11-dione), a major bioactive androgen. Catalyzes the conversion of 11beta-hydroxyandrostenedione (11beta-hydroxyandrost-4-ene-3,17-dione) to 11-ketoandrostenedione (androst-4-ene-3,11,17-trione), which can be further metabolized to 11-ketotestosterone.
| Product Name: | Human HSD11B2 Recombinant Protein (N-His) |
| SKU: | GXRP03448 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | HSD11B2, 11-beta-HSD type II, 11-beta-HSD, 11-DH2, Short chain dehydrogenase/reductase family 9C member 3, Corticosteroid 11-beta-dehydrogenase isozyme 2, SDR9C3, NAD-dependent 11-beta-hydroxysteroid dehydrogenase, 11-beta-hydroxysteroid dehydrogenase type II, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-HSD2, HSD11K, 11-HSD type II |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly106-Arg405 |
| Calculated MW: | 35.15 kDa |
| UniProt: | P80365 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
