Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
The Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit is a powerful tool for the accurate measurement of HMBS levels in human serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.HMBS is a key enzyme involved in heme biosynthesis, playing a crucial role in various physiological processes. Dysregulation of HMBS has been associated with porphyrias, a group of metabolic disorders characterized by the abnormal accumulation of heme precursors.
The Human HMBS ELISA Kit provides researchers with a reliable method for studying HMBS levels in relation to porphyrias and other related conditions.Whether investigating the pathophysiology of porphyrias or exploring potential therapeutic strategies, the Human HMBS ELISA Kit is a valuable resource for researchers looking to deepen their understanding of heme biosynthesis and related disorders. With its high performance and ease of use, this kit is an essential tool for advancing research in the field of molecular biology and medical science.
Product Name:
Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
