Human Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit
The Human Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit is a powerful tool for accurately measuring levels of HPRT1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable asset for various research applications.HPRT1 is an essential enzyme involved in the salvage pathway of purine biosynthesis, playing a critical role in maintaining cellular purine levels and DNA integrity. Dysregulation of HPRT1 has been implicated in various disorders, including Lesch-Nyhan syndrome and certain cancers, making it a crucial biomarker for studying these conditions and identifying potential therapeutic targets.
With the Human HPRT1 ELISA Kit, researchers can accurately quantify HPRT1 levels in human samples, providing valuable insights into its role in disease pathogenesis and potential therapeutic interventions. Trust in this kit for precise and reliable results that can advance your research efforts in the field of molecular biology.
Product Name:
Human Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
