Human IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
The Human IGF-2 (Insulin-Like Growth Factor 2) ELISA Kit is meticulously crafted for the precise quantitative detection of IGF-2 levels in various human biological samples. IGF-2, a potent growth factor, is actively involved in regulating cell growth, proliferation, and differentiation. It plays a crucial role in various biological processes, including metabolic functions, tissue development, and cellular repair mechanisms, making it an essential component of human growth and development. Accurate quantification of IGF-2 is fundamental in elucidating its role in growth regulation, metabolic pathways, and disease progression. This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures, this kit guarantees robust performance, facilitating ease of use in both research and clinical settings. Rely on Assay Genie's IGF-2 ELISA Kit for precise and reliable quantification of this vital growth factor in your research endeavors.
Product Name:
Human IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
SKU:
AEES00073
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
