Human IGF2R (Insulin Like Growth Factor 2 Receptor) CLIA Kit
The Human IGF2R (Insulin-like Growth Factor 2 Receptor) CLIA Kit is specifically designed for the quantitative measurement of IGF2R levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.IGF2R is a key receptor that plays a crucial role in mediating the biological effects of insulin-like growth factor 2 (IGF-2), which is involved in cell growth, differentiation, and survival.
Dysregulation of IGF2R has been linked to various diseases, including cancer and metabolic disorders, highlighting its significance as a potential biomarker for disease prognosis and therapeutic development.With its unparalleled performance and reliability, the Human IGF2R CLIA Kit is an indispensable tool for researchers studying IGF2R biology and its implications in human health and disease. Trust in this kit to provide precise and meaningful data that can further advance your research efforts.
Product Name:
Human IGF2R (Insulin Like Growth Factor 2 Receptor) CLIA Kit
SKU:
HUES00317
Target:
Human IGF2R (Insulin Like Growth Factor 2 Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGF2R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF2R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF2R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGF2R. You can calculate the concentration of Human IGF2R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
89-100
90-105
Average (%)
106
95
96
1:4
Range (%)
91-103
85-96
89-102
Average (%)
97
91
97
1:8
Range (%)
88-103
88-104
84-96
Average (%)
95
95
91
1:16
Range (%)
99-113
94-108
90-101
Average (%)
106
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
419.94
789.36
3128.14
412.07
809.34
3137.23
Standard deviation
43.38
94.72
253.07
43.47
82.8
209.88
C V (%)
10.33
12.0
8.09
10.55
10.23
6.69
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGF2R concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
