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Human IGFBP4 Sf9 Recombinant Protein (RPPB0434)

SKU:
RPPB0434
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22692
Research Area:
Growth Factors & Cytokines
€273
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Description

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Product Name:Human IGFBP4 Sf9 Recombinant Protein
Product Code:RPPB0434
Size:10µg
Species:Human
Target:IGFBP4 Sf9
Synonyms:Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2?m filtered concentrated solution in 20mM Tris-HCl, pH 8.0 and 150mM NaCl.
Solubility:It is recommended to reconstitute the lyophilized Insulin Like Growth Factor Binding Protein-4 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin Like Growth Factor Binding Protein-4 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino Acid Sequence:DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE
Biological Activity:The ED50 is determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells and is 1.0 � 104 IU/mg in the presence of 14 ng/ml of rHuIGF-II.

Insulin-like growth factor-binding protein 4 (IGFBP-4) is a part of the insulin-like growth factor binding protein (IGFBP) family. IGFBP4 includes an IGFBP domain and a thyroglobulin type-I domain. IGFBP4 binds both insulin-like growth factors (IGFs) I and II. IGFBP-4 circulates in the plasma in both glycosylated and non-glycosylated forms. IGFBPs can either inhibit or enhance the biological activities of IGF, or act in an IGF independent manner. IGFBP-4 is consistently inhibits several cancer cells in vivo and in vitro, suggesting that it may function as an apoptotic factor. IGFBP4 is produced by all colon cancer cells. Binding of IGFBP-4 prolongs the half-life of the IGFs and changes their interaction with cell surface receptors.

Insulin Like Growth Factor Binding Protein-4 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 237 amino acids and having a molecular mass of 30kDa. The IGFBP4 is purified by proprietary chromatographic techniques.

UniProt Protein Function:IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: extracellular region; extracellular space

Molecular Function:insulin-like growth factor I binding; insulin-like growth factor II binding; receptor binding

Biological Process: cell proliferation; cellular protein metabolic process; DNA metabolic process; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; skeletal development

NCBI Summary:This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
UniProt Code:P22692
NCBI GenInfo Identifier:124065
NCBI Gene ID:3487
NCBI Accession:P22692.2
UniProt Secondary Accession:P22692,Q5U012, Q9UCL6, A0N9W2, B4E351,
UniProt Related Accession:P22692
Molecular Weight:17,635 Da
NCBI Full Name:Insulin-like growth factor-binding protein 4
NCBI Synonym Full Names:insulin like growth factor binding protein 4
NCBI Official Symbol:IGFBP4��
NCBI Official Synonym Symbols:BP-4; IBP4; IGFBP-4; HT29-IGFBP��
NCBI Protein Information:insulin-like growth factor-binding protein 4
UniProt Protein Name:Insulin-like growth factor-binding protein 4
Protein Family:Insulin-like growth factor-binding protein
UniProt Gene Name:IGFBP4��
UniProt Entry Name:IBP4_HUMAN

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