The Human IgM (Immunoglobulin M) ELISA Kit is specifically designed for the precise measurement of IgM levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research purposes.Immunoglobulin M (IgM) is a primary antibody produced by the immune system in response to infections. Elevated levels of IgM can indicate an active infection, making it a vital biomarker for diagnosing and monitoring various infectious diseases. The Human IgM ELISA Kit provides researchers with a reliable method for detecting and quantifying IgM levels, aiding in the understanding of immune responses and disease progression.Overall, the Human IgM ELISA Kit offers researchers a valuable tool for studying immune responses, infectious diseases, and other conditions where IgM plays a crucial role. Its high sensitivity and specificity make it a trusted choice for accurate and reproducible results in a wide range of applications.
Product Name:
Human IgM (Immunoglobulin M) ELISA Kit
SKU:
HUES02774
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
