The Human IL-1 Beta CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of IL-1 Beta levels in human samples such as serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.IL-1 Beta is a key cytokine involved in inflammatory processes, immune responses, and cell signaling pathways. Dysregulation of IL-1 Beta has been linked to a range of diseases including autoimmune disorders, inflammatory conditions, and infectious diseases.
Therefore, accurate measurement of IL-1 Beta levels is crucial for understanding these diseases and developing potential treatment strategies.The Human IL-1 Beta CLIA Kit from AssayGenie provides researchers with a powerful tool for studying the role of IL-1 Beta in health and disease. Its easy-to-use format and reliable performance make it an indispensable asset for any laboratory conducting research in the field of inflammation and immunology.
Product Name:
Human IL-1 beta CLIA Kit
SKU:
HUES00132
Target:
Human IL-1 beta
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
97-110
102-118
Average (%)
102
104
108
1:4
Range (%)
95-108
97-113
89-101
Average (%)
102
104
96
1:8
Range (%)
86-98
104-116
102-116
Average (%)
92
110
109
1:16
Range (%)
100-115
94-111
92-106
Average (%)
107
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
97-112
104
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.9
77.28
227.64
24.67
80.13
231.61
Standard deviation
2.12
7.86
25.61
2.53
6.8
22.79
C V (%)
8.19
10.17
11.25
10.26
8.49
9.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.
