The Human IL-12 ELISA Kit is specifically designed for the precise measurement of Interleukin-12 (IL-12) levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.IL-12 is a key cytokine involved in regulating immune responses, particularly in the development of T-helper 1 (Th1) cells and enhancing the activity of natural killer cells and T cells. Dysregulation of IL-12 has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By utilizing the Human IL-12 ELISA Kit, researchers can gain valuable insights into the role of IL-12 in immune regulation and disease pathogenesis, ultimately advancing our understanding of these complex processes.
Product Name:
Human IL-12 ELISA Kit
SKU:
HUES01424
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
