The Human IL12-P40 CLIA Kit is specifically designed for the precise measurement of IL12-P40 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research applications.IL12-P40 is a key cytokine involved in the regulation of immune responses, playing a critical role in inflammation and autoimmune diseases. Monitoring IL12-P40 levels can provide valuable insights into the immune system's functioning and potential therapeutic targets for various diseases.
With the Human IL12-P40 CLIA Kit, researchers can accurately measure IL12-P40 levels, leading to a deeper understanding of immune response regulation and disease pathogenesis. This kit is a valuable tool for studying autoimmune disorders, infectious diseases, and inflammatory conditions, offering researchers the ability to uncover new treatment strategies and biomarkers for improved patient care.
Product Name:
Human IL-12 p40 CLIA Kit
SKU:
HUES00134
Target:
Human IL-12 p40
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12 p40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-12 p40. You can calculate the concentration of Human IL-12 p40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
101-120
100-116
Average (%)
91
109
108
1:4
Range (%)
101-118
86-101
87-99
Average (%)
108
93
93
1:8
Range (%)
93-107
103-117
90-105
Average (%)
98
109
97
1:16
Range (%)
103-120
94-106
103-117
Average (%)
110
100
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
92-106
97
Cell culture media (n=5)
100-111
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.18
69.1
293.76
40.45
72.22
264.6
Standard deviation
3.32
6.85
26.97
4.62
8.14
24.08
C V (%)
8.47
9.91
9.18
11.42
11.27
9.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-12 p40 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-12 p40 in samples. No significant cross-reactivity or interference between Human IL-12 p40 and analogues was observed.
