The Human IL-18 ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of IL-18 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for researchers in various fields.IL-18, also known as Interleukin-18, is a proinflammatory cytokine involved in immune responses and inflammatory processes. It has been implicated in various diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and potential therapeutic targets. With its reliable performance and ease of use, the Human IL-18 ELISA Kit from Assaygenie is an essential tool for researchers looking to study the role of IL-18 in health and disease. Visit the link provided for more information on this cutting-edge research tool.
Product Name:
Human IL-18 ELISA Kit
SKU:
HUES01492
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
