The Human IL1R1 (Interleukin-1 receptor type 1) ELISA Kit is specifically designed for the quantitative detection of IL1R1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.IL1R1 is a vital receptor involved in the inflammatory response, playing a key role in mediating the effects of interleukin-1. Dysregulation of IL1R1 signaling has been associated with various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human IL1R1 ELISA Kit provides researchers with a reliable tool to accurately measure IL1R1 levels, contributing to a better understanding of the inflammatory response and its implications in disease pathology.
Product Name:
Human IL-1R1 ELISA Kit
SKU:
HUES02132
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
