The Human IL1RA (IL1F3) ELISA Kit is specially designed for the precise measurement of Interleukin 1 Receptor Antagonist (IL1RA) levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for a variety of research applications.IL1RA is a key cytokine that plays a crucial role in regulating the body's inflammatory response by inhibiting the activity of Interleukin-1 (IL-1). Imbalances in IL1RA levels have been linked to various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.The Human IL1RA (IL1F3) ELISA Kit from AssayGenie provides researchers with a reliable and efficient tool for quantifying IL1RA levels in human samples, aiding in the advancement of scientific understanding and discovery in the field of immunology and inflammatory diseases.
Product Name:
Human IL-1ra/IL-1F3 ELISA Kit
SKU:
HUES01375
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
98-113
84-99
90-104
Average (%)
104
91
98
1:4
Range (%)
91-102
80-89
83-96
Average (%)
97
85
88
1:8
Range (%)
91-102
83-95
83-96
Average (%)
96
88
87
1:16
Range (%)
90-104
83-94
87-102
Average (%)
95
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
92-108
99
Cell Culture Media (n=8)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.4
193.29
879.43
106.95
178.81
951.06
Standard deviation
6.25
11.42
38.96
7.22
11.01
64.29
C V (%)
5.71
5.91
4.43
6.75
6.16
6.76
Uniport ID:
P18510
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed
