The Human IL-23 ELISA Kit is a powerful tool for the precise measurement of IL-23 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IL-23 is a key cytokine involved in the regulation of immune responses, particularly in the development of inflammatory and autoimmune diseases. Elevated levels of IL-23 have been linked to conditions such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease, making it a valuable biomarker for studying these disorders and developing targeted therapies.With its reliable performance and user-friendly protocol, the Human IL-23 ELISA Kit from AssayGenie is an essential tool for researchers seeking to explore the role of IL-23 in health and disease.
Product Name:
Human IL-23 ELISA Kit
SKU:
HUES01393
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
