The Human IL-35 CLIA Kit is a reliable and accurate assay designed for the detection of IL-35 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.IL-35 is a key cytokine involved in regulating immune responses and inflammation, making it a valuable biomarker for studying autoimmune diseases, inflammatory conditions, and cancer.
By measuring IL-35 levels, researchers can gain insights into disease progression and potentially identify new therapeutic targets.Overall, the Human IL-35 CLIA Kit from AssayGenie is a valuable tool for researchers seeking to understand the role of IL-35 in health and disease, offering reliable and accurate measurement of this important cytokine.
Product Name:
Human IL-35 CLIA Kit
SKU:
HUES01275
Target:
Human IL-35
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-35. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-35. You can calculate the concentration of Human IL-35 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
102-114
94-111
Average (%)
98
107
102
1:4
Range (%)
91-104
95-108
99-112
Average (%)
98
102
106
1:8
Range (%)
87-99
83-97
88-101
Average (%)
92
90
94
1:16
Range (%)
96-109
93-106
90-102
Average (%)
102
100
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
101-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.05
59.29
188.36
20.93
61.0
178.66
Standard deviation
1.89
4.4
20.15
2.15
6.81
13.7
C V (%)
8.2
7.42
10.7
10.27
11.16
7.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed.
