The Human IL-35 ELISA Kit is specifically designed for the precise measurement of IL-35 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research purposes.IL-35 is a key cytokine known for its role in regulating immune responses and inflammation. It plays a crucial role in autoimmune diseases, infectious diseases, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing novel treatments. By using the Human IL-35 ELISA Kit, researchers can investigate the intricate mechanisms of IL-35 and its impact on various disease states with confidence and accuracy.
Product Name:
Human IL-35 ELISA Kit
SKU:
HUES03309
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
