The Mouse IL-35 ELISA Kit is specifically designed for the precise measurement of IL-35 levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit offers accurate and reproducible results, making it a valuable tool for researchers in various fields.IL-35 is a key cytokine that plays a critical role in regulating immune responses and inflammation. It has been implicated in a variety of autoimmune diseases, cancer, and infectious diseases, making it an important target for research and therapy development. This ELISA kit provides a simple and reliable method for quantifying IL-35 levels in mouse samples, allowing researchers to better understand the role of this cytokine in disease pathogenesis and potentially identify new therapeutic targets. Order your Mouse IL-35 ELISA Kit today and take your research to the next level.
Product Name:
Mouse IL-35 ELISA Kit
SKU:
MOES01228
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
