The Human IL36A (IL36 Alpha) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of IL36 Alpha levels in human serum, plasma, and cell culture supernatants. With its high specificity, this kit provides accurate and reproducible results, making it an excellent choice for a variety of research applications. IL36 Alpha, also known as Interleukin-36 Alpha, is a pro-inflammatory cytokine that plays a crucial role in the immune response and inflammation regulation. Dysregulation of IL36 Alpha has been implicated in various inflammatory diseases, such as psoriasis and inflammatory bowel disease, making it an essential biomarker for studying and understanding these conditions.
The Human IL36A ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of IL36 Alpha in disease pathogenesis and potential therapeutic targets. Its ease of use, high sensitivity, and specificity make it a valuable resource for studying the complex functions of IL36 Alpha in various research settings.
Product Name:
Human IL-36A/IL-36 alpha ELISA Kit
SKU:
HUFI01718
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
95-103%
91-101%
83-100%
EDTA Plasma (n = 5)
86-99%
83-91%
84-100%
Heparin Plasma (n = 5)
89-102%
84-95%
87-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
90-104
98
EDTA Plasma (n = 5)
86-104
97
Heparin Plasma (n = 5)
86-103
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
