The Human IL7R (Interleukin 7 Receptor Alpha) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of IL7R levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for various research applications. IL7R is a critical component of the interleukin-7 signaling pathway, playing a key role in the regulation of immune cell development, survival, and function. Dysregulation of this pathway has been implicated in various immune-related disorders, including autoimmune diseases and immunodeficiencies.
Therefore, the measurement of IL7R levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies. With its high performance and accuracy, the Human IL7R ELISA Kit is a valuable resource for researchers studying immune system dynamics and exploring novel treatment strategies for immune-related diseases. Get reliable data and meaningful results with this advanced kit from Assay Genie.
Product Name:
Human IL-7R/IL-7 receptor alpha ELISA Kit
SKU:
HUFI00450
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
83-95%
83-101%
80-99%
EDTA Plasma (n = 5)
85-92%
90-99%
86-103%
Heparin Plasma (n = 5)
86-103%
85-98%
85-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-102
93
EDTA Plasma (n = 5)
87-103
94
Heparin Plasma (n = 5)
88-105
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
