Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
The Human Inhibitory Subunit of NF-kappa B Alpha (IkBa) ELISA Kit is a powerful tool designed for the precise measurement of IkBa levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an indispensable asset for a wide array of research endeavors.IkBa is a key regulator of the NF-kappa B signaling pathway, playing a crucial role in the immune response and inflammatory processes.
Dysregulation of IkBa is linked to various diseases, including inflammatory disorders, autoimmune conditions, and cancer. Thus, measuring IkBa levels can provide valuable insights into these pathologies and aid in the development of potential therapeutic interventions.Overall, the Human Inhibitory Subunit of NF-kappa B Alpha (IkBa) ELISA Kit is a valuable resource for researchers seeking to deepen their understanding of IkBa biology and its implications in health and disease.
Product Name:
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Product Code:
HUDL01456
Size:
96 Assays
Target:
IkBa
Synonyms:
NFKBIA, IKB-A, MAD-3, NFKBI, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Alpha, Major histocompatibility complex enhancer-binding protein MAD3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
