The Human INS (Insulin) CLIA Kit is specifically designed for the quantitative detection of insulin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Insulin is a key hormone involved in regulating blood sugar levels and metabolism in the body. Changes in insulin levels can indicate conditions such as diabetes, metabolic disorders, and insulin resistance. Studying insulin levels can help researchers understand these conditions better and develop targeted treatments.The Human INS (Insulin) CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate insulin-related pathways and diseases. With its ease of use and exceptional performance, this kit is a must-have for any laboratory conducting insulin research.
Product Name:
Human INS (Insulin) CLIA Kit
SKU:
HUES00915
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
