Human Intercellular adhesion molecule 3/ICAM3 ELISA Kit
Human Intercellular Adhesion Molecule-3 (ICAM-3) ELISA Kit is a powerful tool for researchers looking to accurately detect and quantify levels of ICAM-3 in human samples. This kit is specially designed for use with human serum, plasma, and cell culture supernatants, providing researchers with a versatile and reliable option for their experiments. ICAM-3 is a critical cell surface glycoprotein involved in mediating cellular adhesion, particularly in immune responses and inflammatory processes. By measuring ICAM-3 levels, researchers can gain valuable insights into the regulation of immune cell interactions and potential therapeutic targets for inflammatory diseases and autoimmune disorders.
The Human ICAM-3 ELISA Kit is known for its high sensitivity and specificity, ensuring precise and reproducible results every time. With its ease of use and accurate detection capabilities, this kit is an essential tool for researchers studying immune response dynamics and developing novel treatments for various diseases. Overall, the Human ICAM-3 ELISA Kit offers a comprehensive solution for researchers looking to investigate the role of ICAM-3 in immune cell adhesion and inflammatory processes in human samples. Its reliability and accuracy make it a valuable asset for a wide range of research applications in immunology, inflammation, and beyond.
Product Name:
Human Intercellular adhesion molecule 3/ICAM3 ELISA Kit
SKU:
HUFI00146
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-104%
87-101%
83-98%
EDTA Plasma (n = 5)
90-101%
84-97%
82-96%
Heparin Plasma (n = 5)
87-104%
90-98%
81-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-105
95
EDTA Plasma (n = 5)
91-105
98
Heparin Plasma (n = 5)
86-99
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
