Human Interleukin 1 Receptor Associated Kinase 3 (IRAK3) ELISA Kit
The Human Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) ELISA Kit is a powerful tool for accurately measuring IRAK3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering dependable and consistent results for various research applications.IRAK3 is a key protein involved in the regulation of immune responses and inflammatory signaling pathways. Dysregulation of IRAK3 has been implicated in various autoimmune diseases, inflammatory conditions, and cancer.
As a valuable biomarker, monitoring IRAK3 levels can provide insights into disease progression and potential therapeutic interventions.Overall, the Human IRAK3 ELISA Kit from Assay Genie is a reliable solution for researchers looking to investigate the role of IRAK3 in health and disease. Its precision and accuracy make it an essential tool for advancing our understanding of immune regulation and inflammatory disorders.
Product Name:
Human Interleukin 1 Receptor Associated Kinase 3 (IRAK3) ELISA Kit
Product Code:
HUDL01547
Size:
96 Assays
Target:
IRAK3
Synonyms:
IRAK-M IRAKM IL-1 receptor-associated kinase M
Detection Method:
Sandwich
Range:
0.781 -50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
