Human Interleukin 11 Receptor Alpha (IL-11Ra) ELISA Kit
The Human Interleukin-11 receptor alpha (IL11RA) ELISA Kit is a specialized tool for the precise measurement of IL11RA levels in human samples including serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity to ensure accurate and reproducible results, making it an invaluable resource for various research applications.IL11RA is a critical receptor involved in the interleukin-11 signaling pathway, playing a key role in various physiological processes such as bone marrow stromal cell proliferation and hematopoiesis.
Dysregulation of IL11RA signaling has been implicated in several diseases including inflammatory disorders, cancer, and bone diseases, highlighting its importance as a potential biomarker for understanding disease mechanisms and exploring therapeutic interventions.With its reliable performance and comprehensive utility, the Human IL11RA ELISA Kit is an indispensable tool for researchers seeking to delve deeper into the intricate role of IL11RA in health and disease.
Product Name:
Human Interleukin 11 Receptor Alpha (IL-11Ra) ELISA Kit
Product Code:
HUDL01462
Size:
96 Assays
Target:
IL11Ra
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
