Human Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit
The Human Interleukin-5 Receptor Alpha (IL5RA) ELISA Kit is a powerful tool for the precise measurement of IL5RA levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and accuracy, this kit ensures dependable and consistent results, making it well-suited for a variety of research applications.IL5RA is a vital receptor involved in the immune response, specifically in mediating the effects of interleukin-5 in allergic and asthmatic conditions. Understanding the role of IL5RA is crucial in elucidating the pathways involved in these diseases and developing targeted therapies.
By accurately quantifying IL5RA levels, researchers can gain valuable insights into the mechanisms underlying allergic and inflammatory responses, paving the way for novel treatments and interventions.Overall, the Human Interleukin-5 Receptor Alpha (IL5RA) ELISA Kit offers researchers a precise and reliable method for studying the role of IL5RA in disease pathogenesis and treatment, ultimately leading to advancements in the field of immunology and personalized medicine.
Product Name:
Human Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
