Description
Human INTS3/SOSS-A Recombinant Protein (C-His)
Human INTS3/SOSS-A Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Thr641-Leu895, with a C-terminal His tag. Predicted molecular weight: 30.75 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Integrator complex subunit 3 (INTS3/SOSS-A) is a ~118 kDa protein. Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. For research use only.
The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS3 is involved in the post-termination step: INTS3 binds INTS7 in the open conformation of integrator complex and prevents the rebinding of Pol II to the integrator after termination cycle. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex.
| Product Name: | Human INTS3/SOSS-A Recombinant Protein (C-His) |
| SKU: | GXRP03299 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SOSS complex subunit A, Int3, Sensor of single-strand DNA complex subunit A, Sensor of ssDNA subunit A, INTS3, SOSS-A, C1orf60, Integrator complex subunit 3, C1orf193 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Thr641-Leu895 |
| Calculated MW: | 30.75 kDa |
| UniProt: | Q68E01 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
