Human Isopentenyl-Diphosphate Delta-Isomerase 1 (IDI1) ELISA Kit
The Human Isopentenyl Diphosphate Delta Isomerase 1 (IDI1) ELISA Kit is a powerful tool for the precise measurement of IDI1 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it suitable for a wide array of research applications.IDI1 is a key enzyme involved in the biosynthesis of isoprenoids, which play critical roles in cellular functions such as membrane structure, protein prenylation, and cell signaling.
Abnormalities in IDI1 expression have been linked to various diseases including cancer, metabolic disorders, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease research and therapeutic development.Get accurate and insightful data on IDI1 levels with the Human Isopentenyl Diphosphate Delta Isomerase 1 ELISA Kit, and gain valuable insights into the intricate mechanisms governing cellular processes and disease pathogenesis.
Product Name:
Human Isopentenyl-Diphosphate Delta-Isomerase 1 (IDI1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
