Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit
The Human KAZAL-Type Serine Protease Inhibitor Domain-Containing Protein 1 (KAZALD1) ELISA Kit is specifically designed for the accurate measurement of KAZALD1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.KAZALD1 is a protein containing a KAZAL domain, which is known for its role in regulating serine protease activity. Studies have shown that KAZALD1 may play a key role in processes such as inflammation, wound healing, and tissue remodeling.
Understanding the expression levels of KAZALD1 can provide valuable insights into various biological processes and disease states.This ELISA kit is a valuable tool for researchers studying the functions of KAZALD1 and its potential implications in various physiological and pathological conditions. With its reliable performance and ease of use, the Human KAZAL-Type Serine Protease Inhibitor Domain-Containing Protein 1 ELISA Kit is a valuable asset for biomedical research.
Product Name:
Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit
Product Code:
HUDL01596
Size:
96 Assays
Target:
KAZALD1
Synonyms:
BONO1, FKSG28, FKSG40
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
