The Human KL (Klotho) ELISA Kit is a highly reliable tool for the precise measurement of Klotho levels in human biological samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides accurate and consistent results, making it an excellent choice for various research applications.Klotho is a key protein that plays a critical role in regulating aging and age-related diseases by modulating multiple signaling pathways. Dysregulation of Klotho has been linked to various health conditions, including kidney disease, cardiovascular disease, and cognitive decline. The Human KL ELISA Kit allows researchers to investigate the role of Klotho in these diseases and explore its potential as a therapeutic target.
Product Name:
Human KL (Klotho) ELISA Kit
SKU:
HUES03445
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
