Description
Human LCAT Recombinant Protein (N-His)
Human LCAT Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Phe25-Glu440, with an N-terminal His tag. Predicted molecular weight: 49.38 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Phosphatidylcholine-sterol acyltransferase (LCAT) is a ~49 kDa protein. Central enzyme in the extracellular metabolism of plasma lipoproteins. For research use only.
Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels.
| Product Name: | Human LCAT Recombinant Protein (N-His) |
| SKU: | GXRP00535 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Platelet-activating factor acetylhydrolase, Phosphatidylcholine-sterol acyltransferase, Phospholipid-cholesterol acyltransferase, PAF acetylhydrolase, LCAT, 1-alkyl-2-acetylglycerophosphocholine esterase, Lecithin-cholesterol acyltransferase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Phe25-Glu440 |
| Calculated MW: | 49.38 kDa |
| UniProt: | P04180 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
