The Human Lectin Mannose-Binding 2 (LMAN2) ELISA Kit is a powerful tool for detecting LMAN2 levels in human biological samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reliable results, making it suitable for a variety of research applications.LMAN2 is a lectin protein that plays a critical role in mediating the binding of glycoproteins to the Golgi apparatus, essential for proper protein trafficking and secretion.
Dysregulation of LMAN2 has been linked to various diseases, including metabolic disorders and neurodegenerative conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human Lectin Mannose-Binding 2 (LMAN2) ELISA Kit offers researchers a valuable tool for studying the role of LMAN2 in health and disease, providing insights into its potential as a target for novel treatments.
Product Name:
Human Lectin, Mannose Binding 2 (LMAN2) ELISA Kit
Product Code:
HUDL01726
Size:
96 Assays
Target:
LMAN2
Synonyms:
GP36B, VIP36, C5orf8, Vesicular integral-membrane protein VIP36, Glycoprotein GP36b, Vesicular integral-membrane protein 36
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
