Human Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2) ELISA Kit
The Human Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR-2) ELISA Kit is a highly accurate and reliable tool for detecting LAIR-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.LAIR-2 is a protein involved in immune regulation and modulation of immune responses. It plays a critical role in regulating immune cell function and has been linked to various diseases including autoimmune disorders and cancer.
Studying LAIR-2 levels can provide valuable insights into the immune system's function and potential therapeutic targets for immune-related diseases.Overall, the Human LAIR-2 ELISA Kit is a valuable tool for researchers looking to study immune regulation, immune responses, and the role of LAIR-2 in various diseases. Its high-quality performance and versatility make it an essential component for any laboratory conducting research in the field of immunology.
Product Name:
Human Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2) ELISA Kit
Product Code:
HUDL01670
Size:
96 Assays
Target:
LAIR2
Synonyms:
CD306
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
